Application

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Signaling

This Anti-Tau, AEP-cleaved (N368) Antibody is validated for use in Western Blotting for the detection of AEP-cleaved Tau N-terminal fragment (N368).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Microtubule-associated protein Tau (UniProt P10636; also known as Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau) is encoded by the MAPT (also known as TAU, MAPT1, MTBTL) gene (Gene ID 4137) in human. Neurofibrillary tangles (NFTs), composed of truncated and hyperphosphorylated Tau, are a common feature of aging-related neurodegenerative diseases, including Alzheimer′s disease (AD). In addition to caspases-mediated cleavage at Asp421, Tau can also be cleaved at Asn255 and Asn368 by the lysosomal cysteine proteinase asparagine endopeptidase (AEP). AEP is upregulated in aged brain, as well as n human AD brain and tau P301S–transgenic mice with synaptic pathology and behavioral impairments. AEP-mediated tau cleavage abolishes tau microtubule assembly function, induces tau aggregation and triggers neurodegeneration. AEP knockout or functional blockage in tau P301S mice leads to a reduction of tau hyperphosphorylation, protection against memory loss, and a reduction in tau P301S–triggered pathological and behavioral defects.

Immunogen

KLH-conjugated linear peptide corresponding to the C-terminal end sequence of AEP-cleaved human Tau N-terminal fragment N368

Epitope: C-terminus

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Rabbit polyclonal antiserum with 0.05% sodium azide.

Unpurified

Quality

Evaluated by Western Blotting using purified Tau.

Western Blotting Analysis: A 1:100,000 dilution of this antiserum specifically detected 1 µg of purified aa 1-368 Tau fragment, but not the full-length Tau protein.

Specificity

Specifically recognizes AEP-generated Tau fragments with N368 as the c-termus. Does not react with full-length Tau or Tau fragments with other cleaved c-termus. Uniprot P10636 lists 9 human tau isoforms produced by alternative splicing. The N368 designation was originally assigned to this polyclonal antiserum based on Asp368 of isoform 8 (Tau-F, Tau-4), which is equivalent to N685 of isoform 1 (PNS-tau), N279 of isoform 2 (Fetal-tau), N243 of isoform 3 (Tau-A), N308 of isoform 4 (Tau-B), N337 of isoform 5 (Tau-C, Tau-3), N310 of isoform 6 (Tau-D), N339 of isoform 7 (Tau-E), and N703 of isoform 9 (Tau-G).

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~45 kDa observed. Variable depending on the isoform and size of the target fragments.